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Appl Environ Microbiol. 1982 October; 44(4): 994-995

Application of halophilic nuclease H of Micrococcus varians subsp. halophilus to commercial production of flavoring agent 5'-GMP.

M Kamekura, T Hamakawa and H Onishi

ABSTRACT

RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, and the yield was 805 mg from 5 g of RNA.

Appl Environ Microbiol. 1982 October; 44(4): 994-995




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