This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Alvarez, M E Articles by O'Brien, R T Search for Related Content PubMed PubMed Citation Articles by Alvarez, M E Articles by O'Brien, R T Agricola Articles by Alvarez, M E Articles by O'Brien, R T

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1982 November; 44(5): 1064-1071

Mechanisms of inactivation of poliovirus by chlorine dioxide and iodine.

ABSTRACT

Chlorine dioxide and iodine inactivated poliovirus more efficiently at pH 10.0 than at pH 6.0. Sedimentation analyses of viruses inactivated by chlorine dioxide and iodine at pH 10.9 showed that viral RNA separated from the capsids, resulting in the conversion of virions from 156S structures to 80S particles. The RNAs release from both chlorine dioxide- and iodine-inactivated viruses cosedimented with intact 35S viral RNA. Both chlorine dioxide and iodine reacted with the capsid proteins of poliovirus and changed the pI from pH 7.0 to pH 5.8. However, the mechanisms of inactivation of poliovirus by chlorine dioxide and iodine were found to differ. Iodine inactivated viruses by impairing their ability to adsorb to HeLa cells, whereas chlorine dioxide-inactivated viruses showed a reduced incorporation of [14C]uridine into new viral RNA. We concluded, then, that chlorine dioxide inactivated poliovirus by reacting with the viral RNA and impairing the ability of the viral genome to act as a template for RNA synthesis.

This article has been cited by other articles:

• Thurston-Enriquez, J. A., Haas, C. N., Jacangelo, J., Gerba, C. P. (2005). Inactivation of Enteric Adenovirus and Feline Calicivirus by Chlorine Dioxide. Appl. Environ. Microbiol. 71: 3100-3105 [Abstract] [Full Text]