AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Monot, F.
Right arrow Articles by Gay, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Monot, F.
Right arrow Articles by Gay, R.
Agricola
Right arrow Articles by Monot, F.
Right arrow Articles by Gay, R.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1982 December; 44(6): 1318-1324
Copyright © 1982, American Society for Microbiology. All Rights Reserved.

Acetone and Butanol Production by Clostridium acetobutylicum in a Synthetic Medium

Frédéric Monot, Jean-René Martin, Henri Petitdemange and Robert Gay

1 Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC, 54042 Nancy, Cedex, France, and Université de Nancy I, Laboratoire de Chimie Biologique I, 54037 Nancy, Cedex, France

ABSTRACT

The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by Clostridium acetobutylicum ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO4, 50 to 200 mg/liter; MnSO4, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K+ was supplied in the form of KH2PO4 and K2HPO4); FeSO4, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; para-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter.

Appl Environ Microbiol. 1982 December; 44(6): 1318-1324
Copyright © 1982, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1982 by the American Society for Microbiology. All rights reserved.