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Appl Environ Microbiol. 1982 December; 44(6): 1325-1329
Copyright © 1982, American Society for Microbiology. All Rights Reserved.

Ethanol Inhibition Kinetics of Kluyveromyces marxianus Grown on Jerusalem Artichoke Juice

Chemical and Biochemical Engineering, Faculty of Engineering Science, The University of Western Ontario, London, Ontario N6A 5B9, Canada

ABSTRACT

The kinetics of ethanol inhibition on cell growth and ethanol production by Kluyveromyces marxianus UCD (FST) 55-82 were studied during batch growth. The liquid medium contained 10% (wt/vol) inulin-type sugars derived from an extract of Jerusalem artichoke (Helianthus tuberosus) tubers, supplemented with small amounts of Tween 80, oleic acid, and corn steep liquor. Initial ethanol concentrations ranging from 0 to 80 g/liter in the liquid medium were used to study the inhibitory effect of ethanol on the following parameters: maximum specific growth rate (µ_max), cell and ethanol yields, and sugar utilization. It was found that as the initial ethanol concentration increased from 0 to 80 g/liter, and maximum specific growth rate of K. marxianus cells decreased from 0.42 to 0.09 h^–1, whereas the ethanol and cell yields and sugar utilization remained almost constant. A simple kinetic model was used to correlate the µ_max results and the rates of cell and ethanol production, and the appropriate constants were evaluated.