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Appl Environ Microbiol. 1983 January; 45(1): 297-301
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Growth and Sporulation of Entrapped Bacillus subtilis Cells

¹ Laboratoire de Technologie Enzymatique and Laboratoire de Génétique Microbienne, ² Centre National de la Recherche Scientifique, 60206 Compiègne, France

ABSTRACT

Growing Bacillus subtilis cells were immobilized in K-carrageenan gel beads. Growth and sporulation of entrapped cells were studied by two different methods: cell enumeration and transmission electron microscopy. Immobilized growing cells had a shorter generation time than free cells did, whereas sporulation timing was unchanged. When steady state was reached, cell density was very high in gel beads.