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Appl Environ Microbiol. 1983 April; 45(4): 1178-1182
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Inhibition of Chemoautotrophic Nitrification by Sodium Chlorate and Sodium Chlorite: a Reexamination

Department of Microbiology, Macdonald Campus, McGill University, Ste. Anne de Bellevue, Quebec, Canada H9X IC0

ABSTRACT

The oxidation of NH₄⁺ by Nitrosomonas europaea was insensitive to 10 mM NaClO₃ (sodium chlorate) but was strongly inhibited by NaClO₂ (sodium chlorite; K_i, 2 µM). The oxidation of NO₂^– by Nitrobacter winogradskyi was inhibited by both ClO₃^– and ClO₂^– (K_i for ClO₂^–, 100 µM). N. winogradskyi reduced ClO₃^– to ClO₂^– under both aerobic and anaerobic conditions, and as much as 0.25 mM ClO₂^– was detected in the culture filtrate. In mixed N. europaea-N. winogradskyi cell suspensions, the oxidation of both NH₄⁺ and NO₂^– was inhibited in the presence of 10 mM ClO₃^– after a 2-h lag period, despite the fact that, under these conditions, ClO₂^– was not detected in the filtrate. The data are consistent with the hypothesis that, in mixed culture, NH₄⁺ oxidation is inhibited by ClO₂^– produced by reduction of ClO₃^– by the NO₂^– oxidizer. The use of ClO₃^– inhibition of NO₂^– oxidation in assays of nitrification by mixed populations necessitates cautious interpretation unless it can be shown that the oxidation of NH₄⁺ is not affected.

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