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Appl Environ Microbiol. 1983 June; 45(6): 1722-1726
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

L-Serine Production by a Mutant of Sarcina albida Defective in L-Serine Degradation

Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Kashima, Yodogawa-ku, Osaka, 532 Japan

ABSTRACT

For improved L-serine production, an L-serine dehydratase-defective mutant of Sarcina albida IAM 1012 was obtained. In the mutant, the activities of the enzymes responsible for L-serine production were as high as those in the parent strain, and, at a low glycine concentration, the mutant accumulated L-serine more efficiently than the parent. Under optimum conditions, 21 mg of L-serine per ml accumulated from 100 mg of glycine per ml. L-Serine was isolated from a reaction mixture as L-serine m-xylene-4-sulfonate, and free amino acid was obtained in high yields by use of an ion-exchange resin. Residual glycine was recovered at a yield of 61%.