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Appl Environ Microbiol. 1983 June; 45(6): 1899-1904
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Controlling Morphological Instability of Zymomonas mobilis Strains in Continuous Culture

¹ Weston Research Centre, Toronto, Canada M4W 2L3, and Department of Biochemistry, University of Toronto, Toronto, Canada M5S 1A8²

ABSTRACT

Growth of Zymomonas mobilis ATCC 29191 and CP4 in a continuous stirred tank fermentor resulted in the selection of stable flocculating variants. Factors responsible for enhancing the system pressures selective for the morphological variants were identified. By incorporating some modifications into the design of the fermentor, it was possible to achieve steady-state operation of the chemostat with both wild-type and flocculating strains. Biochemical and microscopic studies were performed to elucidate the mechanism of flocculation in Z. mobilis.