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Appl Environ Microbiol. 1983 June; 45(6): 1956-1959
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Simple Assay for Accurate Determination of [³⁵S]sulfate Reduction Activity

H. Rick Rosser and W. Allan Hamilton

Department of Microbiology, Marischal College, University, Aberdeen, AB9 1AS, United Kingdom

ABSTRACT

A method is described for the assay of [³⁵S]sulfate reduction in which filter paper wicks are used to trap [³⁵S]sulfide. The simplicity of the technique enables large numbers of samples to be conveniently processed. Enhanced sensitivity is achieved since all acid-volatile [³⁵S]sulfides produced during the incubation period are counted. Recovery of radioactivity from added Na₂³⁵S is excellent (mean, 100.1%; standard deviation, 1.81; n = 9) and is unaffected by sulfide concentrations of up to 400 µg per sample. Field trial results with anoxic sediment samples are presented.

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