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Appl Environ Microbiol. 1983 July; 46(1): 233-236

Paper replication method for isolation of radiation-sensitive mutants.

ABSTRACT

A filter paper replication system particularly useful for isolation of radiation-sensitive mutants of pigmented bacteria was devised. The fidelity of replication was high. Adhesion between a paper disk and a properly dried master plate provided adequate contact pressure. The replicas arising from this technique constitute a convenient apparatus for general application in isolation of clones sensitive to a discriminating treatment.

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