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Appl Environ Microbiol. 1983 August; 46(2): 344-347
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Enzyme-Linked Immunosorbent Assays for Detection of Xylem-Limited Bacteria: Use of Trinder Reagent {dagger}

Mary Starnes Saunders{ddagger} and William J. French

University of Florida, Agricultural Research Center, Monticello, Florida 32344

ABSTRACT

Quantitation and detection of xylem-limited bacteria with an enzyme-linked immunosorbent assay with a peroxidase conjugate is described. The use of the Trinder reagent (4-aminoantipyrine) allows the determination of extremely small quantities of peroxidase with no precipitate formation or inactivation of the enzyme by H2O2. Comparison of the enzyme-linked immunosorbent assay method with microscopic and histochemical tests for the presence of the phony peach disease bacterium in 9-year-old "June Gold" peach trees gave comparable results. The peroxidase conjugate with the Trinder reagent is more sensitive than the alkaline phosphatase conjugate typically used for enzyme-linked immunosorbent assay quantitation.

FOOTNOTES

{ddagger} Present address: Center for Viticultural Science and Small Farm Development, Division of Agricultural Science, Florida A&M University, Tallahassee, FL 32307.

{dagger} Florida Agricultural Experiment Station Journal Series no. 4756.

Appl Environ Microbiol. 1983 August; 46(2): 344-347
Copyright © 1983, American Society for Microbiology. All Rights Reserved.





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Copyright © 1983 by the American Society for Microbiology. All rights reserved.