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Appl Environ Microbiol. 1983 August; 46(2): 484-490
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of Methanomicrobium paynteri sp. nov., a Mesophilic Methanogen Isolated from Marine Sediments {dagger}

Christopher J. Rivard{ddagger}, J. Michael Henson, Michael V. Thomas and Paul H. Smith

Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611

ABSTRACT

A new mesophilic methanogenic bacterial species isolated from marine sediments collected in the Cayman Islands is described. Cells are small rods occuring singly without filaments, are not motile, and do not possess flagella. Colonies are semitransparent and off-white in color. After 2 weeks of incubation at 37°C colonies are 1 to 2 mm in size, circular, and have entire edges. Only hydrogen-carbon dioxide is a substrate for growth and methane formation. Cells can tolerate a variety of organic secondary buffers (bicarbonate-CO2 being the primary buffer). Cells do not require yeast extract or Trypticase, but do require acetate, for growth. The optimum growth temperature is 40°C. The optimum sodium concentration is 0.15 M. The optimum pH for growth is 7.0. The minimum generation time is 4.8 h. The DNA base composition is 44.9 mol% guanine plus cytosine. The name Methanomicrobium paynteri is proposed in honor of M. J. B. Paynter. The type strain is G-2000 (=ATCC 33997, =DSM 2545).

FOOTNOTES

{ddagger} Present address: Solar Energy Research Institute, Golden, CO 80401.

{dagger} Florida Agricultural Experiment Station Journal Series no. 4447.

Appl Environ Microbiol. 1983 August; 46(2): 484-490
Copyright © 1983, American Society for Microbiology. All Rights Reserved.





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Copyright © 1983 by the American Society for Microbiology. All rights reserved.