This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Campbell, L. Articles by Iacono, V. J. Search for Related Content PubMed PubMed Citation Articles by Campbell, L. Articles by Iacono, V. J. Agricola Articles by Campbell, L. Articles by Iacono, V. J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1983 September; 46(3): 553-559
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Identification and Enumeration of Marine Chroococcoid Cyanobacteria by Immunofluorescence

¹ Marine Sciences Research Center and Department of Periodontics, ² State University of New York at Stony Brook, Stony Brook, New York 11794

ABSTRACT

We used an indirect immunofluorescence technique to permit the identification and enumeration of individual or closely related strains of chroococcoid cyanobacteria of the general Synechococcus and Synechocystis in natural seawater samples. Antisera directed against each of five strains (two phycoerythrin-containing Synechococcus strains, two phycocyanin-containing Synechococcus strains, and one Synechocystis strain) were produced and tested for cross-reactions with cultures of a variety of cyanobacteria and representatives of other algae and bacteria. Each antiserum was relatively specific. The observed cross-reactions occurred between strains that were isolated from similar oceanic environments. We were able, therefore, to apply this technique to field samples. Preliminary results for April to December 1982 in Great South Bay, New York, show that Synechocystis populations are present only during spring and summer, phycocyanin-containing Synechococcus strains are only a minor component in the spring and summer, and phycoerythrin-containing Synechococcus populations become significant in summer and remain so until late fall or winter.

This article has been cited by other articles:

• Fuller, N. J., Marie, D., Partensky, F., Vaulot, D., Post, A. F., Scanlan, D. J. (2003). Clade-Specific 16S Ribosomal DNA Oligonucleotides Reveal the Predominance of a Single Marine Synechococcus Clade throughout a Stratified Water Column in the Red Sea. Appl. Environ. Microbiol. 69: 2430-2443 [Abstract] [Full Text]  
• Weinbauer, M. G., Höfle, M. G. (1998). Distribution and Life Strategies of Two Bacterial Populations in a Eutrophic Lake. Appl. Environ. Microbiol. 64: 3776-3783 [Abstract] [Full Text]