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Appl Environ Microbiol. 1983 December; 46(6): 1247-1251
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Chemically Defined Medium for Cultivation of Several Epiphytic and Phytopathogenic Spiroplasmas

I.-M. Lee and R. E. Davis

1 Department of Botany, University of Maryland, College Park, Maryland 20742 and Plant Virology Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 207052*

ABSTRACT

A chemically defined medium, LD82, was formulated for in vitro cultivation of spiroplasmas. Medium LD82 supported good growth for four epiphytic and insect-pathogenic spiroplasmas, Spiroplasma floricola 23-6T, Spiroplasma sp. strain SR3, Spiroplasma sp. strain brevi, and Spiroplasma sp. strain AS576, and of the phytopathogenic spiroplasmas Spiroplasma citri Maroc R8A2T and PC1. Titers of all six strains grown in defined medium LD82 reached 2.0 x 109 to 6.0 x 109 CFU/ml of culture. All spiroplasma strains tested formed colonies readily on agar medium LD82. None of the spiroplasmas formed typical fried-egg colonies. All formed diffuse colonies, but the forms of colonies differed somewhat among the spiroplasma strains. In preliminary studies of nutritional requirements, phospholipids slightly enhanced the growth of the epiphytic and insect-pathogenic strains in medium LD82 and were found essential for good growth of S. citri.

Appl Environ Microbiol. 1983 December; 46(6): 1247-1251
Copyright © 1983, American Society for Microbiology. All Rights Reserved.



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