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Appl Environ Microbiol. 1983 December; 46(6): 1339-1344
Copyright © 1983, American Society for Microbiology. All Rights Reserved.

Analysis of Poly-ß-Hydroxybutyrate in Rhizobium japonicum Bacteroids by Ion-Exclusion High-Pressure Liquid Chromatography and UV Detection

Department of Biochemistry and Interdisciplinary Plant Biochemistry-Physiology Group, University of Missouri-Columbia, Columbia, Missouri 65211

ABSTRACT

Ion-exclusion high-pressure liquid chromatography (HPLC) was used to measure poly-ß-hydroxybutyrate (PHB) in Rhizobium japonicum bacteroids. The products in the acid digest of PHB-containing material were fractionated by HPLC on Aminex HPX-87H ion-exclusion resin for organic acid analysis. Crotonic acid formed from PHB during acid digestion was detected by its intense absorbance at 210 nm. The Aminex-HPLC method provides a rapid and simple chromatographic technique for routine analysis of organic acids. Results of PHB analysis by Aminex-HPLC were confirmed by gas chromatography and spectrophotometric analysis.

This is contribution 9422 from the Agricultural Experiment Station of the University of Missouri-Columbia.

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