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Appl Environ Microbiol. 1984 February; 47(2): 436-438

Use of bacteriophage P1 as a vector for Tn5 insertion mutagenesis.

M Quinto and R A Bender

ABSTRACT

Infection of a strain lysogenic for bacteriophage P1 CM with P1::Tn5 followed by simultaneous selection for the chloroamphenicol resistance associated with the resident prophage and the kanamycin resistance associated with Tn5 results in a large number of independent Tn5 insertion mutations. This superinfection-selection protocol is a fast, easy, and safe way to isolate null mutations in enteric bacteria without generating unwanted cryptic mutations elsewhere in the genome.

Appl Environ Microbiol. 1984 February; 47(2): 436-438






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