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Appl Environ Microbiol. 1984 March; 47(3): 513-518
Copyright © 1984, American Society for Microbiology. All Rights Reserved.

Disinfection of Water with Quaternary Ammonium Salts Insolubilized on a Porous Glass Surface

¹ Department of Fermentation Technology, Faculty of Engineering, Osaka University, 2-1 Yamadaoka, Suita-shi, Osaka 565, and Department of Applied Chemistry, Technical College of Tokushima University, Tokushima 770, ² Japan

ABSTRACT

Insoluble quaternary ammonium salts bound to porous glass showed antibacterial activity. An agent designated as G₁₂, which had a dodecyl alkyl chain, was selected for some antibacterial tests on comparison of it with the agent reported previously. The antibacterial activity of G₁₂ toward Escherichia coli was mainly due to the adsorption of cells and therefore gradually decreased during continuous treatment of a cell suspension. The lost G₁₂ activity was completely recovered by washing with ethanol, and the activity of refreshed G₁₂ decreased in the same manner as that of fresh G₁₂. The lost activity was, however, always recovered only by ethanol treatment. This indicated that G₁₂ might interact with cells more strongly by means of a hydrophobic force than an electrostatic one. The antimicrobial spectrum showed that G₁₂ was effective against not only bacteria but also yeasts.

^* Corresponding author.