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Appl Environ Microbiol. 1984 June; 47(6): 1343-1345
Copyright © 1984, American Society for Microbiology. All Rights Reserved.

Selective Inhibition by 2-Bromoethanesulfonate of Methanogenesis from Acetate in a Thermophilic Anaerobic Digestor

Department of Microbiology, Stocking Hall, Cornell University, Ithaca, New York 14853

ABSTRACT

The effects of 2-bromoethanesulfonate, an inhibitor of methanogenesis, on metabolism in sludge from a thermophilic (58°C) anaerobic digestor were studied. It was found from short-term experiments that 1 µmol of 2-bromoethanesulfonate per ml completely inhibited methanogenesis from ¹⁴CH₃COO^–, whereas 50 µmol/ml was required for complete inhibition of ¹⁴CO₂ reduction. When 1 µmol of 2-bromoethanesulfonate per ml was added to actively metabolizing sludge which was then incubated for 24 h. it caused a 60% reduction in methanogenesis and a corresponding increase in acetate accumulation; at 50 µmol/ml it caused complete inhibition of methanogenesis and accumulation of acetate. H₂, and ethanol.

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