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Appl Environ Microbiol. 1984 June; 47(6): 1358-1359
Copyright © 1984, American Society for Microbiology. All Rights Reserved.

Role of N-Acetylgalactosamine-Specific Protein in Trapping of Nematodes by Arthrobotrys oligospora

Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854

ABSTRACT

An N-acetylgalactosamine-specific protein was purified from mycelial homogenates of the nematode-trapping fungus Arthrobotrys oligospora by using affinity chromatography. The molecular weight of the protein was estimated at 22,000 by its comparative mobility on sodium dodecyl sulfate-polyacrylamide slab gels. Pretreatment of nematodes with the purified protein reduced entrapment, indicating a role for the sugar-binding protein in recognition and capture of prey by the fungus.

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