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Appl Environ Microbiol. 1984 August; 48(2): 280-284

Characterization of Bacillus subtilis DSM704 and its production of 1-deoxynojirimycin.

D C Stein, L K Kopec, R E Yasbin and F E Young

ABSTRACT

A Bacillus subtilis strain, DSM704, was characterized by genetic means, and its production of a human intestinal sucrase inhibitor, 1-deoxynojirimycin, was described. Synthesis of this compound is detected concomitant with the detection of heat-resistant spores. The amount of 1-deoxynojirimycin produced is highly dependent on the carbon source, with growth on substrates metabolized via glycolysis giving the greatest amount of production (up to 1 mg/ml). 1-Deoxynojirimycin appears to be nonmetabolizable by the producing strain in that it cannot serve as a sole carbon or nitrogen source.


Appl Environ Microbiol. 1984 August; 48(2): 280-284







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