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Appl Environ Microbiol. 1984 October; 48(4): 719-725
Copyright © 1984, American Society for Microbiology. All Rights Reserved.

Metabolism of Trimethylamine, Choline, and Glycine Betaine by Sulfate-Reducing and Methanogenic Bacteria in Marine Sediments

I. C. Darling Center, University of Maine, Walpole, Maine 04573, and Department of Microbiology, University of Maine, Orono, Maine 04469

ABSTRACT

The response of methanogenesis and sulfate reduction to trimethylamine, choline, and glycine betaine was examined in surface sediments from the intertidal region of Lowes Cove, Maine. Addition of these substrates markedly stimulated methanogenesis in the presence of active sulfate reduction, whereas addition of other substrates, including glucose, acetate, and glycine, had no effect on methane production. Sulfate reduction was stimulated simultaneously with methanogenesis by the various quaternary amines and all other substrates examined. Incubation of exogenous trimethylamine, choline, or glycine betaine with either bromoethane sulfonic acid or sodium molybdate was used to establish pathways of degradation of the substrates. Methanogenesis dominated the metabolism of trimethylamine, although limited nonmethanogenic activity, perhaps by sulfate-reducing bacteria, was observed. Acetate was oxidized primarily by sulfate reducers. Both choline and glycine betaine were fermented stoichiometrically to acetate and trimethylamine; apparently, neither substrate could be utilized directly by methanogens or sulfate reducers, and the activities of fermenters, methanogens, and sulfate reducers were all required to effect complete mineralization. These observations support the hypothesis that the presence of quaternary amines can mediate the coexistence of sulfate reduction and methanogenesis in marine surface sediments; they also implicate methanogens in the nitrogen cycle of marine sediments containing quaternary amines.

Contribution 171 from the I. C. Darling Center and 84-03 from the Maine Benthic Oceanography Group.

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