This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bilinski, C A Articles by Stewart, G G Search for Related Content PubMed PubMed Citation Articles by Bilinski, C A Articles by Stewart, G G Agricola Articles by Bilinski, C A Articles by Stewart, G G

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1984 October; 48(4): 813-817

Morphological and genetic effects of benomyl on polyploid brewing yeasts: isolation of auxotrophic mutants.

ABSTRACT

An enrichment procedure after ethyl methanesulfonate mutagenesis and exposure to the fungicide benomyl yielded mutants auxotrophic for several amino acids from two polyploid Saccharomyces spp. Benomyl treatment was found to have a marked morphological effect on polyploid Saccharomyces cerevisiae, causing cells to adopt a characteristic doublet cell morphology in which buds are nearly as large as the parent cells. Experiments in which nuclear division was monitored in benomyl-induced doublet cells by Giemsa nuclear staining demonstrated an unusual sequence of cytological events which culminated in the formation of binucleate parental and mononucleate bud components. The frequency of formation of doublet and binucleate parent cells was found to depend on the strain employed and the benomyl concentration administered.

This article has been cited by other articles:

• Hashimoto, S., Ogura, M., Aritomi, K., Hoshida, H., Nishizawa, Y., Akada, R. (2005). Isolation of Auxotrophic Mutants of Diploid Industrial Yeast Strains after UV Mutagenesis. Appl. Environ. Microbiol. 71: 312-319 [Abstract] [Full Text]