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Appl Environ Microbiol. 1985 March; 49(3): 556-562
Copyright © 1985, American Society for Microbiology. All Rights Reserved.

Influence of Manganese on Growth of a Sheathless Strain of Leptothrix discophora

Lee F. Adams and William C. Ghiorse^*

Department of Microbiology, Cornell University, Ithaca, New York 14853

ABSTRACT

Mn²⁺ exerted various effects on the growth of Leptothrix discophora strain SS-1 in batch cultures depending on the concentration added to the medium. Concentrations of 0.55 to 5.5 µM Mn²⁺, comparable to those in the environment from which strain SS-1 was isolated, decreased cell yield and prolonged stationary-phase survival, but did not affect growth rate. Elevated concentrations of 55 to 910 µM Mn²⁺ also decreased cell yield and prolonged survival, but growth rate was decreased as well. The addition of 1,820 µM Mn²⁺ caused a decline in cell numbers followed by an exponential rise after 80 h of incubation, indicating the development of a population of cells resistant to Mn²⁺ toxicity. When 360 µM Mn²⁺ or less was added to growth flasks, Mn²⁺ was oxidized to manganese oxide (MnO_x, where x is 2), which appeared as brown particles in the medium. Quantification of Mn oxidation during growth of cultures to which 55 µM Mn²⁺ was added showed that nearly all of the Mn²⁺ was oxidized by the beginning of the stationary phase of growth (15 to 25 h). This result suggested that the decrease in cell yield observed at low and moderate concentrations of Mn²⁺ was related to the formation of MnO_x, which may have bound cationic nutrients essential to the growth of SS-1. The addition of excess Fe³⁺ to cultures containing 55 µM Mn²⁺ increased cell yield to levels near those found in cultures with no added Mn²⁺, indicating that iron deprivation by MnO_x was at least partly responsible for the decreased cell yield.

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FOOTNOTES

^* Corresponding author.

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Appl Environ Microbiol. 1985 March; 49(3): 556-562
Copyright © 1985, American Society for Microbiology. All Rights Reserved.

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