This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Monticello, D J Articles by Finnerty, W R Search for Related Content PubMed PubMed Citation Articles by Monticello, D J Articles by Finnerty, W R Agricola Articles by Monticello, D J Articles by Finnerty, W R

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1985 April; 49(4): 761-764

Plasmid-borne Tn5 insertion mutation resulting in accumulation of gentisate from salicylate.

D J Monticello, D Bakker, M Schell and W R Finnerty

ABSTRACT

Plasmid-borne Tn5 insertion mutants of a Pseudomonas species which accumulated 2,5-dihydroxybenzoate (gentisate) following growth on 2-hydroxybenzoate (salicylate) were obtained from a pool of mutants that were unable to grow on naphthalene. One such mutant was characterized further. The ability of this mutant to oxidize gentisate was 100-fold less than the ability of a Nah+ Sal+ strain harboring the unmutagenized plasmid, although both strains oxidized and grew on salicylate. These bacteria were presumably able to metabolize salicylate via catechol, since they possessed an inducible, plasmid-encoded catechol 2,3-dioxygenase. Our results suggest that there is an alternate, plasmid-encoded route of salicylate degradation via gentisate and that some plasmid-associated relationship between this pathway and naphthalene oxidation exists.

---

Appl Environ Microbiol. 1985 April; 49(4): 761-764

This article has been cited by other articles:

• Feng, J., Che, Y., Milse, J., Yin, Y.-J., Liu, L., Ruckert, C., Shen, X.-H., Qi, S.-W., Kalinowski, J., Liu, S.-J. (2006). The Gene ncgl2918 Encodes a Novel Maleylpyruvate Isomerase That Needs Mycothiol as Cofactor and Links Mycothiol Biosynthesis and Gentisate Assimilation in Corynebacterium glutamicum. J. Biol. Chem. 281: 10778-10785 [Abstract] [Full Text]  
• Shen, X.-H., Jiang, C.-Y., Huang, Y., Liu, Z.-P., Liu, S.-J. (2005). Functional Identification of Novel Genes Involved in the Glutathione-Independent Gentisate Pathway in Corynebacterium glutamicum. Appl. Environ. Microbiol. 71: 3442-3452 [Abstract] [Full Text]  
• Zhou, N.-Y., Al-Dulayymi, J., Baird, M. S., Williams, P. A. (2002). Salicylate 5-Hydroxylase from Ralstonia sp. Strain U2: a Monooxygenase with Close Relationships to and Shared Electron Transport Proteins with Naphthalene Dioxygenase. J. Bacteriol. 184: 1547-1555 [Abstract] [Full Text]  
• Zhou, N.-Y., Fuenmayor, S. L., Williams, P. A. (2001). nag Genes of Ralstonia (Formerly Pseudomonas) sp. Strain U2 Encoding Enzymes for Gentisate Catabolism. J. Bacteriol. 183: 700-708 [Abstract] [Full Text]