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Appl Environ Microbiol. 1985 April; 49(4): 836-841

Transfer of plasmids pBR322 and pBR325 in wastewater from laboratory strains of Escherichia coli to bacteria indigenous to the waste disposal system.

M A Gealt, M D Chai, K B Alpert and J C Boyer

ABSTRACT

Laboratory strains of Escherichia coli containing plasmid pBR325 (or pBR322) were coincubated with a mobilizer strain of E. coli (containing the conjugative plasmid R100-1) and a recipient strain of bacteria. Bacterial strains isolated from raw wastewater or a plasmid-free E. coli laboratory strain served as recipients. Transfer of the pBR plasmid into the recipient strain occurred during a 25-h coincubation in either L broth or sterilized wastewater; transfer frequencies were several orders of magnitude lower in wastewater. After the coincubation, recipients exhibited both plasmid-encoded phenotypic characteristics and an altered plasmid profile, as shown by agarose gel electrophoresis of purified plasmid DNA.

Appl Environ Microbiol. 1985 April; 49(4): 836-841




This article has been cited by other articles:

  • Hoffmann, A., Thimm, T., Dröge, M., Moore, E. R. B., Munch, J. C., Tebbe, C. C. (1998). Intergeneric Transfer of Conjugative and Mobilizable Plasmids Harbored by Escherichia coli in the Gut of the Soil Microarthropod Folsomia candida (Collembola). Appl. Environ. Microbiol. 64: 2652-2659 [Abstract] [Full Text]  


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