This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mittelman, M W Articles by Geesey, G G Search for Related Content PubMed PubMed Citation Articles by Mittelman, M W Articles by Geesey, G G Agricola Articles by Mittelman, M W Articles by Geesey, G G

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1985 April; 49(4): 846-851

Copper-binding characteristics of exopolymers from a freshwater-sediment bacterium.

M W Mittelman and G G Geesey

ABSTRACT

Copper-binding activity by exopolymers from adherent cells of a freshwater-sediment bacterium was demonstrated by a combination of equilibrium dialysis and flameless atomic absorption spectrometry. Crude, cell-free exopolymer preparations containing protein and polysaccharide components bound up to 37 nmol of Cu per mg (dry weight). A highly purified exopolysaccharide preparation bound up to 253 nmol of Cu per mg of carbohydrate. The conditional stability constant for the crude exopolymer-Cu complex was 7.3 X 10(8). This value was similar to those obtained for Cu complexes formed with humic acids and xanthan, an exopolysaccharide produced by Xanthomonas campestris. Studies conducted at copper concentrations, pHs, and temperatures found in sediments from which the bacterium was isolated indicated that the exopolymers were capable of binding copper under natural conditions.

---

Appl Environ Microbiol. 1985 April; 49(4): 846-851

This article has been cited by other articles:

• Quaranta, D., McCarty, R., Bandarian, V., Rensing, C. (2007). The Copper-Inducible cin Operon Encodes an Unusual Methionine-Rich Azurin-Like Protein and a Pre-Q0 Reductase in Pseudomonas putida KT2440. J. Bacteriol. 189: 5361-5371 [Abstract] [Full Text]  
• Southam, G., Saunders, J. A. (2005). The Geomicrobiology of Ore Deposits. Economic Geology 100: 1067-1084 [Abstract] [Full Text]  
• Zhou, L. X., Zhou, S. G., Zhan, X. H. (2004). Sorption and Biodegradability of Sludge Bacterial Extracellular Polymers in Soil and Their Influence on Soil Copper Behavior. J. Environ. Qual. 33: 154-162 [Abstract] [Full Text]  
• Teitzel, G. M., Parsek, M. R. (2003). Heavy Metal Resistance of Biofilm and Planktonic Pseudomonas aeruginosa. Appl. Environ. Microbiol. 69: 2313-2320 [Abstract] [Full Text]  
• Davey, M. E., O'toole, G. A. (2000). Microbial Biofilms: from Ecology to Molecular Genetics. Microbiol. Mol. Biol. Rev. 64: 847-867 [Abstract] [Full Text]