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Appl Environ Microbiol. 1985 April; 49(4): 852-857

Application of flow cytometry to detection and characterization of Legionella spp.

R L Tyndall, R E Hand Jr, R C Mann, C Evans and R Jernigan

ABSTRACT

Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps related to infectivity, in Legionella populations.


Appl Environ Microbiol. 1985 April; 49(4): 852-857




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