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Appl Environ Microbiol. 1985 May; 49(5): 1142-1145
Copyright © 1985, American Society for Microbiology. All Rights Reserved.

Comparison of Methane Production Rate and Coenzyme F420 Content of Methanogenic Consortia in Anaerobic Granular Sludge {dagger}

Jan Dolfing{ddagger},* and Jan-Willem Mulder

Department of Microbiology, Agricultural University, 6703 CT Wageningen, The Netherlands

ABSTRACT

The coenzyme F420 content of granular sludge grown on various substrates and substrate combinations was measured, and the potential of the sludge to form methane (maximum specific methane production rate) from hydrogen, formate, acetate, propionate, and ethanol was determined. The F420 content varied between 55 nmol g of volatile suspended solids (VSS)–1 for sludge grown on acetate and 796 nmol g of VSS–1 for sludge grown on propionate. The best correlation was found between the F420 content and the potential activity for methane formation from formate; almost no correlation, however, was found with acetate as the test substrate. The ratio between the potential methanogenic activities (qCH4) of sludges grown on various substrates and their F420 content was in general highest for formate (48.2 µmol of CH4 µmol of F420–1 min–1) and lowest for propionate (6.9 µmol of CH4 µmol of F420–1 min–1) as test substrates. However, acetate-grown granular sludge with acetate as test substrate showed the highest ratio, namely, 229 µmol of CH4 µmol of F420–1 min–1. The data presented indicate that the F420 content of methanogenic consortia can be misleading for the assessment of their potential acetoclastic methanogenic activity.

FOOTNOTES

* Corresponding author.

{ddagger} {ddagger} Present address: Department of Microbiology and Public Health, Michigan State University, East Lansing, MI 48824.

{dagger} Reprint requests should be addressed to A. J. B. Zehnder, Department of Microbiology, Agricultural University, 6703 CT Wageningen, The Netherlands.

Appl Environ Microbiol. 1985 May; 49(5): 1142-1145
Copyright © 1985, American Society for Microbiology. All Rights Reserved.





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