This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wadowsky, R M
Right arrow Articles by Yee, R B
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wadowsky, R M
Right arrow Articles by Yee, R B
Agricola
Right arrow Articles by Wadowsky, R M
Right arrow Articles by Yee, R B

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1985 May; 49(5): 1197-1205

Effect of temperature, pH, and oxygen level on the multiplication of naturally occurring Legionella pneumophila in potable water.

R M Wadowsky, R Wolford, A M McNamara and R B Yee

ABSTRACT

A water culture containing naturally occurring Legionella pneumophila and associated microbiota was maintained in the laboratory by serially transferring the culture in tap water which had been sterilized by membrane filtration. Successful maintenance of the water culture depended upon transferring the culture when the growth of L. pneumophila was in the late-exponential to early-stationary phase. The water culture was used as a source of naturally occurring bacteria to determine some of the parameters which affect the multiplication of L. pneumophila in tap water. Naturally occurring L. pneumophila multiplied at a temperature between 25 and 37 degrees C, at pH levels of 5.5 to 9.2, and at concentrations of dissolved oxygen of 6.0 to 6.7 mg/liter. Multiplication did not occur in tap water which contained less than 2.2 mg of dissolved oxygen per liter. An association was observed between the multiplication of L. pneumophila and the non-Legionellaceae bacteria which were also present in the water culture. The method of preserving naturally occurring L. pneumophila and associated microbiota may facilitate studies on the symbiosis of L. pneumophila with other microorganisms.

Appl Environ Microbiol. 1985 May; 49(5): 1197-1205




This article has been cited by other articles:

  • Soderberg, M. A., Dao, J., Starkenburg, S. R., Cianciotto, N. P. (2008). Importance of Type II Secretion for Survival of Legionella pneumophila in Tap Water and in Amoebae at Low Temperatures. Appl. Environ. Microbiol. 74: 5583-5588 [Abstract] [Full Text]  
  • Soderberg, M. A., Cianciotto, N. P. (2008). A Legionella pneumophila Peptidyl-Prolyl cis-trans Isomerase Present in Culture Supernatants Is Necessary for Optimal Growth at Low Temperatures. Appl. Environ. Microbiol. 74: 1634-1638 [Abstract] [Full Text]  
  • Wullings, B. A., van der Kooij, D. (2006). Occurrence and Genetic Diversity of Uncultured Legionella spp. in Drinking Water Treated at Temperatures below 15{degrees}C. Appl. Environ. Microbiol. 72: 157-166 [Abstract] [Full Text]  
  • Costa, J., Tiago, I., da Costa, M. S., Verissimo, A. (2005). Presence and Persistence of Legionella spp. in Groundwater. Appl. Environ. Microbiol. 71: 663-671 [Abstract] [Full Text]  
  • Kuiper, M. W., Wullings, B. A., Akkermans, A. D. L., Beumer, R. R., van der Kooij, D. (2004). Intracellular Proliferation of Legionella pneumophila in Hartmannella vermiformis in Aquatic Biofilms Grown on Plasticized Polyvinyl Chloride. Appl. Environ. Microbiol. 70: 6826-6833 [Abstract] [Full Text]  
  • Soderberg, M. A., Rossier, O., Cianciotto, N. P. (2004). The Type II Protein Secretion System of Legionella pneumophila Promotes Growth at Low Temperatures. J. Bacteriol. 186: 3712-3720 [Abstract] [Full Text]  
  • States, S. J., Conley, L. F., Kuchta, J. M., Wolford, R. S., Wadowsky, R. M., Yee, R. B. (1989). Chlorine, pH, and Control of Legionella in Hospital Plumbing Systems. JAMA 261: 1882-1883 [Abstract]  
  • Helms, C. M., Massanari, R. M., Wenzel, R. P., Pfaller, M. A., Moyer, N. P., Hall, N., Legionella Monitoring Committee, , Streed, S., Johnson, W., Hausler, W. J. Jr, Wmtermeyer, L. A. (1988). Legionnaires' Disease Associated With a Hospital Water System: A Five-Year Progress Report on Continuous Hyperchlorination. JAMA 259: 2423-2427 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»