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Appl Environ Microbiol. 1985 June; 49(6): 1432-1435
Copyright © 1985, American Society for Microbiology. All Rights Reserved.

Conserved Nodulation Genes in Rhizobium meliloti and Rhizobium trifolii

Robert F. Fisher, Janice K. Tu and Sharon R. Long*

Department of Biological Sciences, Stanford University, Stanford, California 94305

ABSTRACT

Plasmids which contained wild-type or mutated Rhizobium meliloti nodulation (nod) genes were introduced into Nod–R. trifolii mutants ANU453 and ANU851 and tested for their ability to nodulate clover. Cloned wild-type and mutated R. meliloti nod gene segments restored ANU851 to Nod+, with the exception of nodD mutants. Similarly, wild-type and mutant R. meliloti nod genes complemented ANU453 to Nod+, except for nodCII mutants. Thus, ANU851 identifies the equivalent of the R. meliloti nodD genes, and ANU453 specifies the equivalent of the R. meliloti nodCII genes. In addition, cloned wild-type R. trifolii nod genes were introduced into seven R. meliloti Nod– mutants. All seven mutants were restored to Nod+ on alfalfa. Our results indicate that these genes represent common nodulation functions and argue for an allelic relationship between nod genes in R. meliloti and R. trifolii.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1985 June; 49(6): 1432-1435
Copyright © 1985, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Roche, P., Maillet, F., Plazanet, C., Debelle, F., Ferro, M., Truchet, G., Prome, J.-C., Denarie, J. (1996). The common nodABC genes of Rhizobium meliloti are host-range determinants. Proc. Natl. Acad. Sci. USA 93: 15305-15310 [Abstract] [Full Text]  
  • Philip-Hollingsworth, S., Orgambide, G. G., Bradford, J. J., Smith, D. K., Hollingsworth, R. I., Dazzo, F. B. (1995). Mutation or Increased Copy Number of nodE Has No Effect on the Spectrum of Chitolipooligosaccharide Nod Factors Made By Rhizobiumleguminosarum bv. trifolii. J. Biol. Chem. 270: 20968-20977 [Abstract] [Full Text]  
  • Sharma, S B, Signer, E R (1990). Temporal and spatial regulation of the symbiotic genes of Rhizobium meliloti in planta revealed by transposon Tn5-gusA.. Genes Dev. 4: 344-356 [Abstract]  
  • Fisher, R F, Egelhoff, T T, Mulligan, J T, Long, S R (1988). Specific binding of proteins from Rhizobium meliloti cell-free extracts containing NodD to DNA sequences upstream of inducible nodulation genes.. Genes Dev. 2: 282-293 [Abstract]  
  • Burn, J., Rossen, L., Johnston, A.W.B. (1987). Four classes of mutations in the nodD gene of Rhizobium leguminosarum biovar. viciae that affect its ability to autoregulate and/or activate other nod genes in the presence of flavonoid inducers. Genes Dev. 1: 456-464 [Abstract]  


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