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Appl Environ Microbiol. 1985 June; 49(6): 1478-1481
Copyright © 1985, American Society for Microbiology. All Rights Reserved.

Selective Isolation of Bacillus sphaericus from Soil by Use of Acetate as the Only Major Source of Carbon

Julie Massie{dagger}, Graham Roberts{ddagger} and Peter J. White*

Department of Microbiology, The University, Western Bank, Sheffield S10 2TN, United Kingdom

ABSTRACT

A simple chemically defined medium containing sodium acetate (37 or 74 mM) as the only major source of carbon was inoculated with soil from various locations. The bacteria which grew most rapidly at 30°C were almost entirely strains of Bacillus sphaericus and Arthrobacter species. Pasteurization of the soils made the medium selective for B. sphaericus. Several new strains were isolated, and the peptidoglycans of their cell walls were examined.

FOOTNOTES

* Corresponding author.

{dagger} Present address: Sheffield Public Health Laboratory, Northern General Hospital, Sheffield S5 7AU, United Kingdom.

{ddagger} Present address: Department of Biochemistry, Cambridge University, Cambridge CB2 1QW, United Kingdom.

Appl Environ Microbiol. 1985 June; 49(6): 1478-1481
Copyright © 1985, American Society for Microbiology. All Rights Reserved.





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