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Appl Environ Microbiol. 1985 July; 50(1): 120-124
Copyright © 1985, American Society for Microbiology. All Rights Reserved.

Effect of Reactor Turbulence on the Binding-Protein-Mediated Aspartate Transport System in Thin Wastewater Biofilms

T. Taylor Eighmy* and P. L. Bishop

Department of Civil Engineering, University of New Hampshire, Durham, New Hampshire 03824

ABSTRACT

This research documents an effect of reactor turbulence on the ability of gram-negative wastewater biofilm bacteria to actively transport L-aspartate via a binding-protein-mediated transport system. Biofilms which were not preadapted to turbulence and which possessed two separate and distinct aspartate transport systems (systems 1 and 2) were subjected to a turbulent flow condition in a hydrodynamically defined closed-loop reactor system. A shear stress treatment of 3.1 N · m–2 for 10 min at a turbulent Reynolds number (Re = 11,297) inactivated the low-affinity, high-capacity binding-protein-mediated transport system (system 2) and resolved the high-affinity, low-capacity membrane-bound proton symport system (system 1). The Kt and Vmax values for the resolved system were statistically similar to Kt and Vmax values for system 1 when system 2 was inactivated either by osmotic shock or arsenate, two treatments which are known to inactivate binding-protein-mediated transport systems. We hypothesize that shear stress disrupts system 2 by deforming the outer membranes of the firmly adhered gram-negative bacteria.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1985 July; 50(1): 120-124
Copyright © 1985, American Society for Microbiology. All Rights Reserved.





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Copyright © 1985 by the American Society for Microbiology. All rights reserved.