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Appl Environ Microbiol. 1985 July; 50(1): 152-159
Copyright © 1985, American Society for Microbiology. All Rights Reserved.

Regulation of ß-1, 4-Glucosidase Expression by Candida wickerhamii

S. N. Freer* and R. W. Detroy

Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604

ABSTRACT

Candida wickerhamii NRRL Y-2563 expressed ß-glucosidase activity (3 to 8 U/ml) constitutively when grown aerobically in complex medium containing either glycerol, succinate, xylose, galactose, or cellobiose as the carbon source. The addition of a high concentration of glucose (>75 g/liter) repressed ß-glucosidase expression (<0.3 U/ml); however, this yeast did produce ß-glucosidase when the initial glucose concentration was ≤50 g/liter. When grown aerobically in medium containing glucose plus the above-listed carbon sources, diauxic utilization of the carbon source was observed and the expression of ß-glucosidase was glucose repressed. Surprisingly, glucose repression did not occur when the cells were grown anaerobically. When grown anaerobically in medium containing 100 g of glucose per liter, C. wickerhamii produced 6 to 9 U of enzyme per ml and did not demonstrate diauxic utilization of glucose-cellobiose mixtures. To our knowledge, this is the first report of apparent derepression of a glucose-repressed enzyme by anaerobiosis.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1985 July; 50(1): 152-159
Copyright © 1985, American Society for Microbiology. All Rights Reserved.





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