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Appl Environ Microbiol. 1985 July; 50(1): 169-171

Construction of a cosmid clone library of Pseudomonas syringae pv. phaseolicola and isolation of genes by functional complementation.

M Ehrenshaft and D Mills

ABSTRACT

A genomic library constructed from a wild-type strain of Pseudomonas syringae pv. phaseolicola in the broad-host-range cosmid vector pVK102 was used to isolate wild-type genes by complementation of Tn5-induced auxotrophic mutants. Selection pressure was required for maintenance of the vector and members of the library in strains of P. syringae.

Appl Environ Microbiol. 1985 July; 50(1): 169-171






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