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Appl Environ Microbiol. 1985 July; 50(1): 27-30
Copyright © 1985, American Society for Microbiology. All Rights Reserved.

Genetic Recombination in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium

Department of Chemical, Biological, and Environmental Sciences, Oregon Graduate Center, Beaverton, Oregon 97006-1999

ABSTRACT

Heterokaryons made from auxotrophic strains of the lignin-degrading basidiomycete Phanerochaete chrysosporium were induced to fruit. The isolation of wild-type and double-mutant phenotypes from these crosses indicated that genetic recombination had occurred. Cytological studies demonstrated that more than 90% of the basidiospores from the wild-type and auxotrophic strains and from forced heterokaryons were binucleate. Colonies of the wild-type strain of P. chrysosporium arising from single, predominantly uninucleate conidia were all capable of producing fruit bodies and basidiospores.

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• Sollewijn Gelpke, M. D., Mayfield-Gambill, M., Lin Cereghino, G. P., Gold, M. H. (1999). Homologous Expression of Recombinant Lignin Peroxidase in Phanerochaete chrysosporium. Appl. Environ. Microbiol. 65: 1670-1674 [Abstract] [Full Text]