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Appl Environ Microbiol. 1985 August; 50(2): 229-237
Copyright © 1985, American Society for Microbiology. All Rights Reserved.

Muramic Acid Measurements for Bacterial Investigations in Marine Environments by High-Pressure Liquid Chromatography

Toru Mimura* and Jean-Claude Romano

Centre Océanologique de Marseille (Unité Associée no. 41 du Centre National de la Recherche Scientifique), Station Marine d'Endoume, 13007 Marseille, France

ABSTRACT

Muramic acid, a constituent of procaryotic cell walls, was assayed by high-pressure liquid chromatography in samples from several marine environments (water column, surface microlayer, and sediment) and a bacterial culture. It is used as a microbial biomass indicator. The method gave a good separation of muramic acid from interfering compounds with satisfactory reproducibility. A pseudomonad culture had a muramic acid content of 4.7 x 10–10 to 5.3 x 10–10 µg per cell during growth. In natural water samples, highly significant relationships were found between muramic acid concentrations and bacterial numbers for populations of 108 to 1011 cells per liter. The muramic acid content in natural marine water decreased from 5.3 x 10–10 to 1.6 x 10–10 µg per cell with increasing depth. In coastal sediments exposed to sewage pollution, concentrations of muramic acid, ATP, organic carbon, and total amino acids displayed a parallel decrease with increasing distance from the sewage outlet. Advantages of muramic acid measurement by high-pressure liquid chromatography are its high sensitivity and reduction of preparation steps, allowing a short time analysis.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1985 August; 50(2): 229-237
Copyright © 1985, American Society for Microbiology. All Rights Reserved.





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