This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hino, T Articles by Russell, J B Search for Related Content PubMed PubMed Citation Articles by Hino, T Articles by Russell, J B Agricola Articles by Hino, T Articles by Russell, J B

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1985 December; 50(6): 1368-1374

Effect of reducing-equivalent disposal and NADH/NAD on deamination of amino acids by intact rumen microorganisms and their cell extracts.

ABSTRACT

When mixed rumen microorganisms were incubated in media containing the amino acid source Trypticase, both monensin and carbon monoxide (a hydrogenase inhibitor) decreased methane formation and amino acid fermentation. Both of the methane inhibitors caused a significant increase in the ratio of intracellular NADH to NAD. Studies with cell extracts of rumen bacteria and protozoa indicated that the ratio of NADH to NAD had a marked effect on the deamination of reduced amino acids, in particular branched-chain amino acids. Deamination was inhibited by the addition of NADH and was stimulated by methylene blue, an agent that oxidizes NADH. Neutral and oxidized amino acids were unaffected by NADH. The addition of small amounts of 2-oxoglutarate greatly enhanced the deamination of branched-chain amino acids and indicated that transamination via glutamate dehydrogenase was important. Formation of ammonia from glutamate was likewise inhibited by NADH. These experiments indicated that reducing-equivalent disposal and intracellular NADH/NAD ratio were important effectors of branched-chain amino acid fermentation.

This article has been cited by other articles:

• Calsamiglia, S., Busquet, M., Cardozo, P. W., Castillejos, L., Ferret, A. (2007). Invited Review: Essential Oils as Modifiers of Rumen Microbial Fermentation. J DAIRY SCI 90: 2580-2595 [Abstract] [Full Text]  
• Busquet, M., Calsamiglia, S., Ferret, A., Carro, M. D., Kamel, C. (2005). Effect of Garlic Oil and Four of its Compounds on Rumen Microbial Fermentation. J DAIRY SCI 88: 4393-4404 [Abstract] [Full Text]  
• Busquet, M., Calsamiglia, S., Ferret, A., Cardozo, P. W., Kamel, C. (2005). Effects of Cinnamaldehyde and Garlic Oil on Rumen Microbial Fermentation in a Dual Flow Continuous Culture. J DAIRY SCI 88: 2508-2516 [Abstract] [Full Text]  
• Mohammed, N., Ajisaka, N., Lila, Z. A., Hara, K., Mikuni, K., Hara, K., Kanda, S., Itabashi, H. (2004). Effect of Japanese horseradish oil on methane production and ruminal fermentation in vitro and in steers. J ANIM SCI 82: 1839-1846 [Abstract] [Full Text]  
• Farmer, C. G., Cochran, R. C., Nagaraja, T. G., Titgemeyer, E. C., Johnson, D. E., Wickersham, T. A. (2004). Ruminal and host adaptations to changes in frequency of protein supplementation. J ANIM SCI 82: 895-903 [Abstract] [Full Text]