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Appl Environ Microbiol. 1986 January; 51(1): 206-208

Rapid physical mapping by transposon Tn5 mutagenesis to localize the cloned yeast ILV2 gene.

T K Van Dyk, S C Falco and R A LaRossa

ABSTRACT

A rapid method for Tn5 mutagenesis of cloned genes on multicopy plasmids was used to map a yeast ILV2 mutant allele encoding a sulfometuron methyl-resistant acetolactate synthase. Twenty-one of 40 independent Tn5 insertions were within the 5.6-kilobase-pair cloned segment. Of these, seven adjacent transposition events inactivated the sulfometuron methyl resistance determinant, localizing the ILV2 gene to a minimum 1.4-kilobase-pair region.

Appl Environ Microbiol. 1986 January; 51(1): 206-208






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