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Appl Environ Microbiol. 1986 February; 51(2): 238-243
Copyright © 1986, American Society for Microbiology. All Rights Reserved.

Solid Medium for Culturing Black Smoker Bacteria at Temperatures to 120°C

² Chesapeake Bay Institute, The Johns Hopkins University, Shady Side, Maryland 20764, ¹ and School of Oceanography, WB-10, University of Washington, Seattle, Washington 98195²

ABSTRACT

A solid, highly thermostable medium, based on the new gelling agent GELRITE, was devised to facilitate the culturing of extremely thermophilic microorganisms from submarine hydrothermal vents. The medium remained solid at temperatures to 120°C at vapor pressures and hydrostatic pressures to 265 atm. It proved useful to its maximum tested limits in isolating colonies of black smoker bacteria from hydrothermal fluids recently collected at the Juan de Fuca Ridge in the Pacific Ocean.

^* Corresponding author.