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Appl Environ Microbiol. 1986 March; 51(3): 481-486
Copyright © 1986, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of Coproporphyrin Produced by Four Subspecies of Bacillus thuringiensis

R. L. Harms, D. R. Martinez and V. M. Griego^*

Department of Biological Sciences, Wichita State University, Wichita, Kansas 67208

ABSTRACT

It was found by using spectrophotometric, spectrofluorometric, and high-pressure liquid chromatography that four subspecies of Bacillus thuringiensis produce coproporphyrin. The porphyrin isomer was identified as coproporphyrin I for B. thuringiensis subsp. kurstaki (HD1). The porphyrin was isolated both from spores and from a variety of spent growth media. The quantity of porphyrin released by each Bacillus subspecies differed. The rank order of porphyrin production follows: B. thuringiensis subsp. kurstaki HD1 > B. thuringiensis subsp. thuringiensis HD27 > B. thuringiensis subsp. thuringiensis HD41 > B. thuringiensis subsp. darmstadiensis HD199.

^* Corresponding author.

Present address: Department of Chemistry, University of New Mexico, Albuquerque, NM 87131.