AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kamicker, B. J.
Right arrow Articles by Brill, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kamicker, B. J.
Right arrow Articles by Brill, W. J.
Agricola
Right arrow Articles by Kamicker, B. J.
Right arrow Articles by Brill, W. J.

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1986 March; 51(3): 487-492
Copyright © 1986, American Society for Microbiology. All Rights Reserved.

Identification of Bradyrhizobium japonicum Nodule Isolates from Wisconsin Soybean Farms

Barbara J. Kamicker{dagger} and Winston J. Brill*,{ddagger}

Department of Bacteriology and Center for Studies of Nitrogen Fixation, University of Wisconsin, Madison, Wisconsin 53706

ABSTRACT

One-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis was a more discriminating method than serotyping for identifying strains of Bradyrhizobium japonicum. Analysis of 543 nodule isolates from southeastern Wisconsin soybean farms revealed that none of the isolates were formed by any of the inoculant strains supplied by either of two inoculant companies. Twenty-nine indigenous strains and six inoculant strains were identified. Strain 61A76, the most competitive indigenous strain, formed 21% of the nodules. Indigenous strains 3030, 3058, 0336, and 3052 formed 15, 11, 9, and 9% of the nodules, respectively. These predominant strains were not associated with a particular soybean cultivar, soil type, or farm location.

FOOTNOTES

* Corresponding author.

{dagger} Present address: Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA 02543.

{ddagger} Present address: Agracetus, 8520 University Green, Middleton, WI 53562.

Appl Environ Microbiol. 1986 March; 51(3): 487-492
Copyright © 1986, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 1986 by the American Society for Microbiology. All rights reserved.