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Appl Environ Microbiol. 1986 May; 51(5): 1127-1129

Selective-enrichment procedure for isolation of Listeria monocytogenes from fecal and biologic specimens.

M P Doyle and J L Schoeni

ABSTRACT

A selective-enrichment procedure (SEP) was developed to isolate Listeria monocytogenes from fecal and biologic specimens. This procedure was compared with direct plating with McBride listeria agar and 2-, 4-, and 8-week cold-enrichment procedures in recovering L. monocytogenes from mouse fecal, liver, and brain specimens. Although the SEP occasionally did not isolate the organism from specimens proved positive by the other procedures, the SEP isolated L. monocytogenes from about two and five times as many specimens as the cold-enrichment and direct-plating procedures, respectively.

Appl Environ Microbiol. 1986 May; 51(5): 1127-1129






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