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Appl Environ Microbiol. 1986 July; 52(1): 197-199

Modified plasmid isolation method for Clostridium perfringens and Clostridium absonum.

I Roberts, W M Holmes and P B Hylemon

ABSTRACT

A rapid plasmid isolation procedure for Clostridium perfringens and C. absonum is described. The ratio of culture volume to lysis buffer volume was found to be crucial for efficient plasmid isolation. The method can be scaled up, without difficulty, for large-scale plasmid preparation.

Appl Environ Microbiol. 1986 July; 52(1): 197-199




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