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Appl Environ Microbiol. 1986 July; 52(1): 98-100
Copyright © 1986, American Society for Microbiology. All Rights Reserved.

Demethylation of Veratrole by Cytochrome P-450 in Streptomyces setonii

John B. Sutherland

Institute of Wood Research and the BioSource Institute, Michigan Technological University, Houghton, Michigan 49931

ABSTRACT

The actinomycete Streptomyces setonii 75Vi2 demethylates vanillic acid and guaiacol to protocatechuic acid and catechol, respectively, and then metabolizes the products by the ß-ketoadipate pathway. UV spectroscopy showed that this strain could also metabolize veratrole (1,2-dimethoxybenzene). When grown in veratrole-containing media supplemented with 2,2'-dipyridyl to inhibit cleavage of the aromatic ring, S. setonii accumulated catechol, which was detected by both liquid chromatography and gas chromatography. Reduced cell extracts from veratrole-grown cultures, but not sodium succinate-grown cultures, produced a carbon monoxide difference spectrum with a peak at 450 nm that indicated the presence of soluble cytochrome P-450. Addition of veratrole or guaiacol to oxidized cell extracts from veratrole-grown cultures produced difference spectra that indicated that these compounds were substrates for cytochrome P-450. My results suggest that S. setonii produces a cytochrome P-450 that is involved in the demethylation of veratrole and guaiacol to catechol, which is then catabolized by the ß-ketoadipate pathway.

Appl Environ Microbiol. 1986 July; 52(1): 98-100
Copyright © 1986, American Society for Microbiology. All Rights Reserved.



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Copyright © 1986 by the American Society for Microbiology. All rights reserved.