This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zeyer, J Articles by Timmis, K N Search for Related Content PubMed PubMed Citation Articles by Zeyer, J Articles by Timmis, K N Agricola Articles by Zeyer, J Articles by Timmis, K N

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1986 August; 52(2): 334-339

Influence of para-substituents on the oxidative metabolism of o-nitrophenols by Pseudomonas putida B2.

ABSTRACT

Pseudomonas putida B2 is able to grow on o-nitrophenol (ONP) as the sole source of carbon and nitrogen. ONP was converted by a nitrophenol oxygenase to nitrite and catechol. Catechol was then attacked by a catechol 1,2-dioxygenase and further degraded through an ortho-cleavage pathway. ONP derivatives which were para-substituted with a methyl-, chloro-, carboxy-, formyl- or nitro-group failed to support growth of strain B2. Relevant catabolic enzymes were characterized to analyze why these derivatives were not mineralized. Nitrophenol oxygenase of strain B2 is a soluble, NADPH-dependent enzyme that is stimulated by magnesium, manganese, and calcium ions. It is active toward ONP, 4-methyl-, 4-chloro-, and to a lesser extent, 4-formyl-ONP but not toward 4-carboxy- or 4-nitro-ONP. In addition, 4-formyl-, 4-carboxy-, and 4-nitro-ONP failed to induce the formation of nitrophenol oxygenase. Catechol 1,2-dioxygenase of strain B2 is active toward catechol and 4-methyl-catechol but only poorly active toward chlorinated catechols. 4-Methyl-catechol is likely to be degraded to methyl-lactones, which are often dead-end metabolites in bacteria. Thus, of the compounds tested, only unsubstituted ONP acts as an inducer and substrate for all of the enzymes of a productive catabolic pathway.

This article has been cited by other articles:

• Xiao, Y., Zhang, J.-J., Liu, H., Zhou, N.-Y. (2007). Molecular Characterization of a Novel ortho-Nitrophenol Catabolic Gene Cluster in Alcaligenes sp. Strain NyZ215. J. Bacteriol. 189: 6587-6593 [Abstract] [Full Text]  
• Perez-Reinado, E., Blasco, R., Castillo, F., Moreno-Vivian, C., Roldan, M. D. (2005). Regulation and Characterization of Two Nitroreductase Genes, nprA and nprB, of Rhodobacter capsulatus. Appl. Environ. Microbiol. 71: 7643-7649 [Abstract] [Full Text]  
• Behrend, C., Heesche-Wagner, K. (1999). Formation of Hydride-Meisenheimer Complexes of Picric Acid (2,4,6-Trinitrophenol) and 2,4-Dinitrophenol during Mineralization of Picric Acid by Nocardioides sp. Strain CB 22-2. Appl. Environ. Microbiol. 65: 1372-1377 [Abstract] [Full Text]  
• James, K. D., Williams, P. A. (1998). ntn Genes Determining the Early Steps in the Divergent Catabolism of 4-Nitrotoluene and Toluene in Pseudomonas sp. Strain TW3. J. Bacteriol. 180: 2043-2049 [Abstract] [Full Text]