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Appl Environ Microbiol. 1986 August; 52(2): 388-389
Copyright © 1986, American Society for Microbiology. All Rights Reserved.

Long-Term Storage of Plant-Pathogenic Bacteria in Sterile Distilled Water

Nicola S. Iacobellis{dagger},* and James E. DEVay

Department of Plant Pathology, University of California, Davis, California 95616

ABSTRACT

This study was made to determine the effectiveness of the preservation of plant-pathogenic bacteria in sterile distilled water. After 20 or 24 years of storage in distilled water, a very high percentage (90 to 92%) of the isolates of Agrobacterium tumefaciens and Pseudomonas spp. were still alive. Moreover, 12 of 13 viable (after 24 years) isolates of P. syringae subsp. syringae maintained their ability to produce syringomycin and were pathogenic to bean seedlings. The water-stored cells of two isolates of P. syringae subsp. syringae, when observed by electron microscopy, were smaller than cells of 24-h-old subcultures of bacterial cells grown in nutrient broth; the water-stored cells appeared plasmolysed with an electron-dense cytoplasm and thickened cell wall.

FOOTNOTES

* Corresponding author.

{dagger} Present address: Istituto Tossine e Micotossine da Parassiti vegetali, CNR, 70126 Bari, Italy.

Appl Environ Microbiol. 1986 August; 52(2): 388-389
Copyright © 1986, American Society for Microbiology. All Rights Reserved.



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Copyright © 1986 by the American Society for Microbiology. All rights reserved.