This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Romero, J Articles by Martín, J F Search for Related Content PubMed PubMed Citation Articles by Romero, J Articles by Martín, J F Agricola Articles by Romero, J Articles by Martín, J F

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1986 October; 52(4): 892-897

Utilization of ornithine and arginine as specific precursors of clavulanic acid.

ABSTRACT

Ornithine and arginine (5 to 20 mM), but not glutamic acid or proline, exerted a concentration-dependent stimulatory effect on the biosynthesis of clavulanic acid in both resting-cell cultures and long-term fermentations of Streptomyces clavuligerus. Ornithine strongly inhibited cephamycin biosynthesis in the same strain. [1-14C]-, [5-14C]-, or [U-14 C] ornithine was efficiently incorporated into clavulanic acid, whereas the incorporation of uniformly labeled glutamic acid was very poor. [U-14C] citrulline were not incorporated at all. Mutant nca-1, a strain that is blocked in clavulanic acid biosynthesis, did not incorporate arginine into clavulanic acid. S. clavuligerus showed arginase activity, converting arginine into ornithine, but not amidinotransferase activity. Both arginase activity and clavulanic acid formation were enhanced simultaneously by supplementing the production medium with 10 mM arginine.

This article has been cited by other articles:

• de la Fuente, A., Martin, J. F., Rodriguez-Garcia, A., Liras, P. (2004). Two Proteins with Ornithine Acetyltransferase Activity Show Different Functions in Streptomyces clavuligerus: Oat2 Modulates Clavulanic Acid Biosynthesis in Response to Arginine. J. Bacteriol. 186: 6501-6507 [Abstract] [Full Text]  
• de la Fuente, A., Lorenzana, L. M., Martin, J. F., Liras, P. (2002). Mutants of Streptomyces clavuligerus with Disruptions in Different Genes for Clavulanic Acid Biosynthesis Produce Large Amounts of Holomycin: Possible Cross-Regulation of Two Unrelated Secondary Metabolic Pathways. J. Bacteriol. 184: 6559-6565 [Abstract] [Full Text]  
• Pérez-Redondo, R., Rodríguez-García, A., Martín, J. F., Liras, P. (1999). Deletion of the pyc Gene Blocks Clavulanic Acid Biosynthesis Except in Glycerol-Containing Medium: Evidence for Two Different Genes in Formation of the C3 Unit. J. Bacteriol. 181: 6922-6928 [Abstract] [Full Text]