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Appl Environ Microbiol. 1987 January; 53(1): 204-207

Expression of cloned monkey metallothionein in Escherichia coli.

Y Murooka and T Nagaoka

ABSTRACT

Expression vectors were constructed in which a cDNA specifying the monkey kidney metallothionein-II (MT-II) was linked directly to the lambda PR promoter. Enhanced expression of MT-II in Escherichia coli was observed when two initiation signals were tandemly linked to the MT-II gene and the lambda cI+ host cells were induced by nalidixic acid.

Appl Environ Microbiol. 1987 January; 53(1): 204-207




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