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Appl Environ Microbiol. 1987 October; 53(10): 2588-2589

Isolation of intact high-molecular-weight DNA by using guanidine isothiocyanate.

J A Lippke, M N Strzempko, F F Raia, S L Simon and C K French

BioTechnica Diagnostics, Cambridge, Massachusetts 02138.

ABSTRACT

A method for obtaining high-molecular-weight chromosomal DNA from Bacteroides intermedius and Bacteroides gingivalis is described. This technique is a modification of the guanidine isothiocyanate isolation procedure for RNA and should be useful for isolating intact DNA from organisms with high endogenous nuclease activity.

Appl Environ Microbiol. 1987 October; 53(10): 2588-2589




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