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Appl Environ Microbiol. 1987 November; 53(11): 2677-2679
Copyright © 1987, American Society for Microbiology. All Rights Reserved.

Effects of Flow Cytometric Analysis on Morphology and Viability of Fragile Phytoplankton {dagger}

Elin M. Haugen, Terry L. Cucci, Clarice M. Yentsch and Lynda P. Shapiro*

Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine 04575

ABSTRACT

We assessed damage done to especially delicate marine phytoplankton cells by passage through a Coulter Epics V flow cytometer. The cells did not distort or lyse after exposure to fluidics or to laser light to 1,000 mW. The cells did sustain damage evidenced by temporary growth rate depressions. The four clones tested eventually resumed control growth rates after growth lags to 48 h.


FOOTNOTES

* Corresponding author.

{dagger} Contribution no. 87013 from Bigelow Laboratory for Ocean Sciences.


Appl Environ Microbiol. 1987 November; 53(11): 2677-2679
Copyright © 1987, American Society for Microbiology. All Rights Reserved.







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Copyright © 1987 by the American Society for Microbiology. All rights reserved.